... is with me.
Two constructs to make.
Two colonies I had.
Each trial a single clone.
I cut them, they cut just the way I hoped.
Each clone was sequenced.
The sequence told me that I have what I need.
Although my hopes were low, I did succeed.
And now the clones are sent into the fire ...
Will they do my bidding?
Will they tell me the truth?
(I feel like Nick Cave)
PS Cloning = make an exact copy, in this case a DNA construct. Actually what I did was construct a a gene on a DNA vector and then introduce the newly made reagent into bacteria so that the cells would copy the novel DNA fragment. I would never clone a human. Promise.
- Log in to post comments
More like this
This the third part of case study where we see what happens when high school students clone and sequence genomic plant DNA. In this last part, we use the results from an automated comparison program to determine if the students cloned any genes at all and, if so, which genes were cloned. (You can…
"Come quickly, Watson," said Sherlock Holmes, "I've been asked to review a mysterious sequence, whose importance I'm only now beginning to comprehend."
The unidentified stranger handed Holmes a piece of paper inscribed with symbols and said it was a map of unparalleled value.
Holmes gazed…
How to win the X PRIZE in genomics
In October, 2006, the X PRIZE foundation announced that second X prize would focus on genomics. The first team to successfully sequence 100 human genomes in 10 days will win $10 million dollars.
And I would venture to guess, that the winning team would also win…
Astute ERV readers have noticed a couple of odd things about my research.
1- How the hell am I cutting and pasting bits of a retroviral (RNA) genome together?
2- How the hell do I have a (seemingly) endless supply of HIV-1 for my experiments?
The answer to both questions is, infectious molecular…
Just a single construct to make.
Hundreds of colonies I had.
Each trial 36 clones.
I cut them, they just didn't cut the way I hoped.
No clone was sequenced.
The sequence would have veryfied that I have not what I need.
Although my hopes were high, I did not succeed.
Never mistake PvuI for PvuII, especially if a BamHI/PvuI and a BamHI/PvuII fragment have the same size and you fill in the ends for blunt end ligation.
And rather do refer to your lab book immediately where it is documented that you have used the wrong enzyme instead of waiting a week before looking it up (due to bad experiences my documentation really improved since my time as a PhD student).
To quote Nick himself:
The ladders of life we scale merrily
Move mysteriously around
So that when you think you're climbing up, man
In fact you're climbing down