DIYbio
cagapakis | September 14, 2010This year's Cambridge iGEM team has made a tiny, wireless lightbulb filled with bioluminescent bacteria! There are two main ways of engineering luminescence in E. coli (I assume these are E. coli, correct me if I'm wrong!). One is to express the luciferase gene from fireflies, which adds ATP and oxygen to the chemical luciferin, producing oxyluciferin and yellow, green, or red light.
Since the lightbulb is blue, this bacteria is probably expressing the Lux operon from Vibrio fischeri, which use their bioluminescence in an awesome underwater symbiosis. From the Cambridge iGEM wiki:
Some…
cagapakis | June 15, 2010The first (and sometimes 3rd, 12th, 25th, 134th...) step of any genetic engineering experiment is often extracting DNA from some organism or another. While novel gene synthesis technology will likely make this procedure obsolete, these days it's still most economical to do it by hand. Extracting DNA from fruits like strawberries has also seen a popular resurgence thanks to groups like DIYbio, with instructions for making DNA shots available online for a fun and nerdy party activity. Today my iGEM team extracted RNA from strawberries and oranges to isolate the genes responsible for strawberry…
cagapakis | May 25, 2010A recent New York Times article tells us that what many people call food allergies are actually simple intolerances, and that allergies are being dangerously overdiagnosed. What is a true food allergy, and what can be done to fix them besides banning peanuts from schools and avoiding foods that make us itchy?
Allergies are caused by an inappropriate immune response to common things in the environment. Usually the offending allergen is a protein that comes from plants or animals like pollen or dander. Instead of the immune system recognizing that these proteins are harmless, it instead…
cagapakis | April 12, 2010I got a lot of interesting responses to my post about DIYbio and how modeling innovation in biotech on computer hacker culture may lead to a science that is less "democratized" than what is being proposed. My friend Adam pointed me to Jaron Lanier's work criticizing the "open" and "free" culture movements online as both unfair and leading to cultural stagnation. While I don't agree with all of Lanier's arguments about the prospects of an open digital culture, he makes a lot of really important points that resonate with my feelings about the future of science based on the open online model, in…
cagapakis | March 24, 2010Happy Ada Lovelace Day! Today we blog to celebrate women in technology and science and remember Ada Lovelace, the woman considered to have written the world's first computer program back in the 1840's.
So to celebrate, here's a clip of an interview with Rita Levi-Montalcini, one of my favorite Sassy Bitches of Scienceâ¢. At age 100, she is the oldest living Nobel laureate, sharp as a tack, still working, and a sassy dresser to boot. Her story is incredible and inspiring (especially in light of what I wrote about yesterday on DIYbio, oppression, and opportunity). Turned away from her position…
cagapakis | March 23, 2010I read R.C. Lewontin's Biology as Ideology: The Doctrine of DNA over the weekend and was struck in particular by one line in his wonderful diatribe against biological determinism and reductionism:
"Intellectuals in their self-flattering wish-fulfillment say that knowledge is power, but the truth is that knowledge further empowers only those who have or can acquire the power to use it."
This is something that was really hard to read at first, especially as someone who is overeducated and clearly spends a lot of time thinking about educating other people about science. But I realized that it…
sporte | January 13, 2010Is there a place for citizen scientists in the world of digital biology?
Many of the citizen science projects that I've been reading about have a common structure. There's a University lab at the top, outreach educators in the middle, and a group of citizens out in the field collecting data.
After the data are collected, they end up in a database somewhere and the University researchers analyze them and write papers. At least that's my impression so far.
It seems to me, that with all kinds of databases out there, on-line, there should be plenty of opportunity for both citizens and student…
sporte | March 16, 2009I guess I put my foot in it when I wrote that Genome Technology article on DIY Bio. I've already gotten a couple of e-mails today and I can see on the Google groups DIY bio section, that I managed to offend some people by suggesting doing biotechnology successfully at home might mean that you actually have to learn some biology.
Funny, huh?
It's like I suggested something heretical. People have to learn how to program to write software, don't they? I don't see that doing biotechnology as a hobby would be any different.
Anyway, here's my response to the DIY google group critics. I've…